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Antimicrobico-resistenza e zoonosi: integrazione tra Real-Time PCR e sequenziamento Nanopore nell’approccio One Health

Bibliografia

 

1. Holmes, A.H.; Moore, L.S.P.; Sundsfjord, A.; Steinbakk, M.; Regmi, S.; Karkey, A.; Guerin, P.J.; Piddock, L.J.V. Understanding the Mechanisms and Drivers of Antimicrobial Resistance. The Lancet 2016, 387, 176–187.

2. Beltz, L.A. Emerging Infectious Diseases, A Guide to Diseases, Causative Agents, and Surveillance - First Edition; Jossey-Bass, 2011; ISBN 978-0470398036.

3. Chokshi, A.; Sifri, Z.; Cennimo, D.; Horng, H. Global Contributors to Antibiotic Resistance. J Glob Infect Dis 2019, 11, 36–42, doi:10.4103/jgid.jgid_110_18.

4. McEwen, S.A.; Collignon, P.J. Antimicrobial Resistance: A One Health Perspective. Microbiol Spectr 2018, 6, doi:10.1128/microbiolspec.arba-0009-2017.

5.         EFSA), E.F.S.A.; (ECDC), E.C. for D.P. and C. The European Union One Health 2022 Zoonoses Report. EFSA Journal 2023, 21, e8442–e8442, doi:https://doi.org/10.2903/j.efsa.2023.8442.

6. Van Boeckel, T.P.; Brower, C.; Gilbert, M.; Grenfell, B.T.; Levin, S.A.; Robinson, T.P.; Teillant, A.; Laxminarayan, R. Global Trends in Antimicrobial Use in Food Animals. Proc Natl Acad Sci U S A 2015, 112, 5649–5654, doi:10.1073/pnas.1503141112.

7. WHO Global Antimicrobial Resistance and Use Surveillance System (GLASS) Report 2022; World Health Organization, 2022; ISBN 9789240062702.

8. Rodríguez-Beltrán, J.; DelaFuente, J.; León-Sampedro, R.; MacLean, R.C.; San Millán, Á. Beyond Horizontal Gene Transfer: The Role of Plasmids in Bacterial Evolution. Nat Rev Microbiol 2021, 19, 347–359, doi:10.1038/s41579-020-00497-1.

9. Didelot, X.; Fraser, C.; Gardy, J.; Colijn, C.; Malik, H. Genomic Infectious Disease Epidemiology in Partially Sampled and Ongoing Outbreaks. Mol Biol Evol 2017, 34, 997–1007, doi:10.1093/molbev/msw275.

10. Holzhauer, M.; Wennink, G.J. Zoonotic Risks of Pathogens from Dairy Cattle and Their Milk-Borne Transmission. J Dairy Res 2023, 90, 325–331, doi:10.1017/S0022029923000730.

11. WHO Global Action Plan on Antimicrobial Resistance; 2015; ISBN 9789241509763.

12. Laupland, K.B.; Valiquette, L. The Changing Culture of the Microbiology Laboratory; 2013; Vol. 24.

13. Zukowska, M. Advanced Methods of Bacteriological Identification in a Clinical Microbiology Laboratory. Journal of Pre-Clinical and Clinical Research 2021, 15, 68–72, doi:10.26444/jpccr/134646.

14. Dung, T.T.N.; Phat, V.V.; Vinh, C.; Lan, N.P.H.; Phuong, N.L.N.; Ngan, L.T.Q.; Thwaites, G.; Thwaites, L.; Rabaa, M.; Nguyen, A.T.K.; et al. Development and Validation of Multiplex Real-Time PCR for Simultaneous Detection of Six Bacterial Pathogens Causing Lower Respiratory Tract Infections and Antimicrobial Resistance Genes. BMC Infect Dis 2024, 24, doi:10.1186/s12879-024-09028-2.

15. Taxt, A.M.; Avershina, E.; Frye, S.A.; Naseer, U.; Ahmad, R. Rapid Identification of Pathogens, Antibiotic Resistance Genes and Plasmids in Blood Cultures by Nanopore Sequencing. Sci Rep 2020, 10, 1–11, doi:10.1038/s41598-020-64616-x.

16. Zhang, W.; Du, P.; Zheng, H.; Yu, W.; Wan, L.; Chen, C. Whole-Genome Sequence Comparison as a Method for Improving Bacterial Species Definition. Journal of General and Applied Microbiology 2014, 60, 75–78, doi:10.2323/jgam.60.75.

17. Yang, Y.; Che, Y.; Liu, L.; Wang, C.; Yin, X.; Deng, Y.; Yang, C.; Zhang, T. Rapid Absolute Quantification of Pathogens and ARGs by Nanopore Sequencing. Science of the Total Environment 2022, 809, 152190, doi:10.1016/j.scitotenv.2021.152190.

18. Zhang, C.; Xiu, L.; Li, Y.; Sun, L.; Li, Y.; Zeng, Y.; Wang, F.; Peng, J. Multiplex PCR and Nanopore Sequencing of Genes Associated with Antimicrobial Resistance in Neisseria Gonorrhoeae Directly from Clinical Samples. Clin Chem 2021, 67, 610–620, doi:10.1093/clinchem/hvaa306.

19. Li, B.; Liu, H.; Wang, W. Multiplex Real-Time PCR Assay for Detection of Escherichia Coli O157:H7 and Screening for Non-O157 Shiga Toxin-Producing E. Coli. BMC Microbiol 2017, 17, 1–13, doi:10.1186/s12866-017-1123-2.

20. FAO; OIE; WHO One Health Joint Plan of Action, 2022–2026; FAO; UNEP; WHO; World Organisation for Animal Health (WOAH) (founded as OIE); 2022;

 

 

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05/05/2026

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